Peptide Lab Testing & Certificates of Analysis
How to read a COA, what HPLC and mass spec actually prove, and our current third-party results.
Why Testing Matters
A peptide vial is a white powder — you can't see purity or identity. Independent lab testing is the only way to know the vial contains the right molecule, at the right purity, without contaminants from synthesis.
The Two Tests That Matter Most
HPLC: Purity
High-performance liquid chromatography separates a sample into its components. The result is a chromatogram: the target peptide should appear as one large peak, and purity is reported as that peak's share of the total area. Look for 98–99% or higher.
Mass Spectrometry: Identity
Mass spec measures molecular weight. The observed mass should match the theoretical mass of the sequence — for example about 1419.5 g/mol for BPC-157. A high-purity sample of the wrong peptide would still pass HPLC, which is why you need both tests.
How to Read a Certificate of Analysis
- Lab name: an independent, identifiable laboratory.
- Lot number: matches the label on your vial.
- Test date: recent, ideally within the last 12 months.
- HPLC purity: with the chromatogram attached.
- Mass spec result: observed vs. theoretical mass.
- Appearance & content: lyophilized white powder; net peptide amount.
Peptide Authority: Current COAs
| Product | Lot · Purity · COA |
|---|---|
| BPC-157 10mg | [LOT#] · [99.x%] · PDF |
| TB-500 10mg | [LOT#] · [99.x%] · PDF |
| GHK-Cu 50mg | [LOT#] · [99.x%] · PDF |
| MOTS-c 10mg | [LOT#] · [99.x%] · PDF |
| CJC-1295 / Ipamorelin | [LOT#] · [99.x%] · PDF |
| Epitalon 10mg | [LOT#] · [99.x%] · PDF |
Need a COA for another lot? Contact us with your order number.
Peptide Testing FAQs
How are research peptides made?
Most research peptides are made by solid-phase peptide synthesis (SPPS), where amino acids are added one at a time to a growing chain. The product is then purified by HPLC and freeze-dried (lyophilized) into a stable powder.
How do I know if a peptide is pure?
Ask for a third-party Certificate of Analysis (COA) matched to the lot number on the vial. It should show HPLC purity (ideally 98–99%+) and mass spectrometry confirming the correct molecular weight.
What is a Certificate of Analysis (COA)?
A COA is a lab report confirming a peptide's identity and purity. A trustworthy COA comes from an independent lab, lists the lot number, test date, HPLC purity and mass spec results, and matches the label on your vial.
What's the difference between HPLC and mass spectrometry?
HPLC measures purity — how much of the sample is the target peptide versus impurities. Mass spectrometry confirms identity — that the molecule has the right molecular weight. You need both to trust a peptide.
How should peptides be stored?
Lyophilized peptides should be kept refrigerated (2–8°C) short-term or frozen (−20°C or colder) long-term, away from light and moisture. Let vials reach room temperature before opening to avoid condensation.